|MIP Technologies AB|
MIPs are economical and fast to produce and are robust and stable under storage. They can be used at elevated temperatures, in organic solvents and at extreme pH values. They also display a higher sample load capacity for small molecules than is typical for immuno-affinity based sorbents. This results in higher recoveries for analytical applications and suitability of using the sorbents for semi-preparative or preparative scale separations. In trace analysis applications, the level of compounds that may leach out from the MIP are extremely low, due to efficient washing procedures and do not interfere with the quantification of the target analyte. Template molecules used are usually analogs that differ sufficiently from the target analytes to avoid co-elution problems. Typically, any leachable compound from the MIP preparation is removed (around 99 %) and is normally not detected during analysis. Thin-layer MIPs give higher surface areas and improved mass transfer. With its new proprietary technology known as ‘Grafting’, in which MIPs are directed to the surface of porous silica particles, Biotage can produce solid phase materials that have large surface areas (up to 1000m2 per gram) and improved mass transfer properties. This is particularly important where separation of similar molecules (eg enantiomers) is required or fast kinetic resolution is desirable. Biotage holds patents in the area of hierarchical imprinting, where the template is immobilized onto a support material during polymerization, whereafter the support is removed. The thereby polymers obtained now have binding sites that are highly accessible to molecules that are much larger than the imprinted template. This is particularly relevant to epitope imprinting of proteins and similar applications. As depicted in the figure, a fragment of the target is sufficient to form a specific cavity into which the whole molecule (eg. protein or peptide) can than bind.
MIPs are designed, synthetic polymeric adsorbents. They are highly crosslinked with predetermined binding site cavities for a particular analyte or a group of structurally related analytes. The selectivity is based on both size exclusion and highly defined chemical interaction sites and utilizes hydrogen bonding, ionic or non-polar van der Waals interactions etc.
In the figure below, a schematic route to the synthesis of MIPs is depicted, where a template molecule directs the interaction of designed functional monomers. After the complex formation, a cross-linking monomer connects the monomers in a rigid network and forms a defined cavity. When the template is washed out, the cavity is able to rebind the same molecule, similar molecules or sometimes unrelated molecules which share a common substructure.
The unique selectivity of our designed resins makes them ideally suited for removal of low level contaminants or extraction of high value desirables from your process. Further, as these resins have minimal impact on the flavor and fragrance of the product, they are ideal for use in food, beverage, flavor and fragrance industries.
MIPs are engineered cross-linked polymers that can exhibit high affinity and selectivity towards a single compound or they can be designed to exhibit ‘Class Selectivity’ for a family of related compounds. MIPs are able to bind analytes even when these are present in complex matrices (e.g. plasma, urine, muscle tissue, food matrices, environmental samples, process solutions etc). An important strength of MIPs is that they are able to bind to trace levels of the target analyte, in the presence of large excess of other compounds that have similar physico-chemical properties.
Unlike most separation particles that exhibit only non-selective interactions, MIP particles have a selective synthetic recognition site (or imprint), which is sterically and chemically complementary to a particular analyte or class of analytes. The interactions mimic antibody or receptor binding and are stronger than interactions obtained with conventional separation materials. A particularly interesting characteristic of MIPs is their ability to achieve high recoveries at low analyte concentrations (see Table 1 below where extraction data for the AFFINILUTE MIP – Clenbuterol are taken from -Shimelis, O., Aurand, C., and Trinh, A., Reporter 25.2). This may be attributed to the spectrum of affinity sites within a given MIP polymer where typically there is a low concentration of high affinity sites.