|Zist Abzar Pajoohan|
Medicine Medical SuppliesDetection Kit
GoldAu Nanoparticle /Nanopowder CAS Number : 7440-57-5
This product is LFT Amphetamine rapid diagnostic kit containing spherical Au nanoparticles with the average size of 20 nm. Due to larger surface area of Au nanoparticles, the substrate can be functionalized by various antibodies, which causes a more sensitive test. Nanoparticles because of their small sizes can move readily through porosities of cellulose to reach the line test. In addition, red color of the colloidal gold is an advantage of that for using in rapid diagnostic test.
Lateral flow tests also known as lateral flow immunochromatographic assays, are simple devices intended to detect the presence (or absence) of a target analyte in sample (matrix) without the need for specialized and costly equipment, though many lab based applications exist that are supported by reading equipment. A simple LFT device consists of four sections (sample pad, conjugate pad, nitrocellulose membrane and absorbent pad) which are laminated into a sheet of plastic backing oderly. There are various possible formats depending on the type of target analyte. The two kinds of format frequently used are competitive assay and sandwich assay. Competitive format is employed most often when testing analytes with low molecular weight or presenting single antigenic determinant. In a competitive format, an analyte-protein conjugate coated on the test zone of a nitrocellulose membrane captures a labeled antianalyte monoclonal antibody complex; allowing colour particle (e.g. colloidal gold) to concentrate and form a visible line on the test zone.Another specific antibody coated on the control line allows the capture of the excess antibody complex. One band colour will therefore be visible in the control zone regardeless of the presence of target analytes (e.g. sulfonamides), confirming correct test development. Conversely, a negative sample will result to the formation of two band colours visible (test line and control line).