Medicine Tissue Engineering Product Number : NCP-HS384-10Scaffold
In vivo, cells exist in three-dimensional aggregate/mass, and interface with the other cell or extra cellular matrix (ECM). In conventional in vitro cell culture method which is easy and has a long history, the cells attach to culture dish and form monolayer. This method have achieved many knowledge in biology. However, it is reported many times that the cells cultured in 2D method are far different from the cells in vivo. The excessive activation of proliferation signal caused by cells attachment to the plastic dish and poor (two-dimensional) interface with the other cells are the main reasons.
Recently, it is well known that cells grown under 3D cell culture technique more closely mimic natural tissues and organs microenvironment than cells grown in 2D. In 3D cell culture, cells form multi-cellular clusters (spheroids) that are driven by cell-cell and cell-matrix contracts, rather than cell-plastic contracts. In this environment, cells can exert forces on one another and can move and migrate as they do in vivo.
3D cell culture methods fall into two major groups. One is culturing in hydrogel sponge, e.g. ECM and agarose (i.e. scaffold type). Another is culturing in the low attachment dish (i.e. scaffold-free type). These methods have merit and demerit, and these are used according to the situation demand.